@Article{SchuchLiSaSaJaCeLo:2015:MoSeMe,
               author = "Schuch, Andr{\'e} P. and Lipinski, Victor M. and Santos, 
                         Maur{\'{\i}}cio B. and Santos, Caroline P. and Jardim, Sinara S. 
                         and Cechin, Sonia Z. and Loreto, Elgion L. S.",
          affiliation = "{Instituto Nacional de Pesquisas Espaciais (INPE)} and 
                         {Universidade Federal de Santa Maria (UFSM)} and {Universidade 
                         Federal de Santa Maria (UFSM)} and {Universidade Federal de Santa 
                         Maria (UFSM)} and {Universidade Federal de Santa Maria (UFSM)} and 
                         {Universidade Federal de Santa Maria (UFSM)} and {Universidade 
                         Federal de Santa Maria (UFSM)}",
                title = "Molecular and sensory mechanisms to mitigate sunlight-induced DNA 
                         damage in treefrog tadpoles",
              journal = "Journal of Experimental Biology",
                 year = "2015",
               volume = "218",
               number = "19",
                pages = "3059--3067",
                month = "Oct.",
             keywords = "Amphibian decline, DNA photoproducts, Nucleotide excision repair, 
                         Photolyases, UV radiation.",
             abstract = "The increased incidence of solar ultraviolet B (UVB) radiation has 
                         been proposed as an environmental stressor, which may help to 
                         explain the enigmatic decline of amphibian populations worldwide. 
                         Despite growing knowledge regarding the UV-induced biological 
                         effects in several amphibian models, little is known about the 
                         efficacy of DNA repair pathways. In addition, little attention has 
                         been given to the interplay between these molecular mechanisms 
                         with other physiological strategies that avoid the damage induced 
                         by sunlight. Here, DNA lesions induced by environmental doses of 
                         solar UVB and UVA radiation were detected in genomic DNA samples 
                         of treefrog tadpoles (Hypsiboas pulchellus) and their DNA repair 
                         activity was evaluated. These data were complemented by monitoring 
                         the induction of apoptosis in blood cells and tadpole survival. 
                         Furthermore, the tadpoles' ability to perceive and escape from UV 
                         wavelengths was evaluated as an additional strategy of 
                         photoprotection. The results show that tadpoles are very sensitive 
                         to UVB light, which could be explained by the slow DNA repair 
                         rates for both cyclobutane pyrimidine dimers (CPDs) and pyrimidine 
                         (6,4) pyrimidone photoproducts (6,4PPs). However, they were 
                         resistant to UVA, probably as a result of the activation of 
                         photolyases during UVA irradiation. Surprisingly, a sensory 
                         mechanism that triggers their escape from UVB and UVA light avoids 
                         the generation of DNA damage and helps to maintain the genomic 
                         integrity. This work demonstrates the genotoxic impact of both UVB 
                         and UVA radiation on tadpoles and emphasizes the importance of the 
                         interplay between molecular and sensory mechanisms to minimize the 
                         damage caused by sunlight.",
                  doi = "10.1242/jeb.126672",
                  url = "http://dx.doi.org/10.1242/jeb.126672",
                 issn = "0022-0949",
             language = "en",
           targetfile = "2015_such.pdf",
        urlaccessdate = "27 abr. 2024"
}